Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA. |
| |
| Authors: | Y Kamio Y Itoh and Y Terawaki |
| |
| Institution: | Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan. |
| |
| Abstract: | RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII iterons, a region immediately upstream of the repA promoter, and a 10-base-pair region located between the most external incII iteron and a GATC box. The protection was less efficient when preheated RepA was used. |
| |
| Keywords: | |
|
|
