合欢花对慢性应激大鼠生长和脑单胺类神经递质含量的影响

为探讨合欢花对慢性应激大鼠生长和脑单胺类神经递质的影响,采用15只大鼠,设置了对照组、应激组和合欢花组3组实验。应激组和合欢花组均接受7天的应激刺激,之后合欢花组再灌胃合欢花10天。实验结束后,取3组大鼠的脑组织,用高效液相色谱法测定高香草酸(HVA)、去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的含量。结果表明,应激组大鼠日增重显著低于对照组(P=0.011);而合欢花组大鼠的日增重极显著高于应激组(P=0.002)。应激组大鼠海马、纹状体和前额叶中的HVA含量与对照组相比,虽有升高的趋势,但无显著差异;两组间的NE、DA和5-HT也无显著差异。合欢花组大鼠海马中的HVA、DA含量明显高于应激组,而前额叶中的多巴胺和5-羟色胺,以及纹状体中的5-羟色胺均明显低于应激组。这表明合欢花对慢性应激引起的大鼠生长受抑有缓解作用,对其脑内单胺类神经递质有调节作用。

Effect of Albizia julibrissin on Growth and Brain Monoamine Neurotransmitters in Chronic-Stressed Rats

The study was conducted to investigate the effects of Albizia julibrissin on growth and brain monoamine neurotransmitters in chronic-stressed rats. Fifteen rats were divided into three groups including the control group, stressed group and Albizia julibrissin treated group. The rats in both the stressed and treated groups were stressed for seven days, and the rats in the treated group were fed with Albizia julibrissin by gavage administration for 10 days after stress. At the conclusion of the experiment, brain tissues were collected from the three groups to analyze the content of homovanillic acid (HVA), norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT). The indices above were determined by high pressure liquid chromatogram (HPLC). The results showed that the daily growth in mass of rats in the stressed group significantly decreased compared with the control group (P=0.011), while the Albizia julibrissin treated group had a higher growth than that of the stressed group (P=0.002). The HVA levels of the hippocampus, striatum and prefrontal lobes in the stressed group were not significantly different from the control group, though there was a tendency for higher levels in the stressed group. The stress had no significant effects on levels of NE, DA and 5-HT. The levels of HVA and DA in the hippocampus in the Albizia julibrissin treated group were significantly higher than those of the stressed group, while DA and 5-HT of the prefrontal lobes and 5-HT of the striatum were significantly lower than the stressed group. These results suggest that Albizia julibrissin alleviates the growth inhibition caused by stress, and regulates the levels of monoamine neurotransmitters of the brain in stressed rats.