| 成年大鼠海马神经前体细胞表达功能性的 L-型钙通道 |
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| 引用本文： | 冯锦丽,胡德辉,陈 明,田映红,施志敏,杨建明,朱心红,李晓文,高天明. 成年大鼠海马神经前体细胞表达功能性的 L-型钙通道[J]. 生物化学与生物物理进展, 2005, 32(9): 822-828 |
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| 作者姓名： | 冯锦丽 胡德辉 陈 明 田映红 施志敏 杨建明 朱心红 李晓文 高天明 |
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| 作者单位： | 第一军医大学解剖学教研室,生理学教研室,广州,510515 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金，教育部长江学者奖励计划 |
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| 摘 要： | 为了建立一种能够获得高纯度成年大鼠海马神经前体细胞(HPCs)的体外贴壁培养方法,并鉴定HPCs上是否存在功能性L-型钙通道,分离Wistar成年大鼠海马组织,制成单细胞悬液,利用无血清培养技术,在添加碱性成纤维细胞生长因子(bFGF)、表皮生长因子(EGF)、N2和B27 supplement的DMEM/F12培养液中进行培养.连续传代,采用细胞免疫荧光法对第六代细胞进行鉴定,呈巢蛋白(nestin)阳性的细胞可达99.9%.把培养高纯度的细胞在分化培养基中诱导分化14天后,表现为神经元和星形胶质细胞的形态,且分别呈Ⅲ型β-微管蛋白(Tujl)阳性和胶质纤维酸性蛋白(GFAP)阳性.细胞免疫荧光和免疫印迹结果显示,HPCs表达L-型钙通道的Cav1.2α1C和Cav1.3α1D亚单位,共聚焦钙成像证明了功能性L-型钙通道的存在,并且利用全细胞膜片钳技术记录到了L-型钙电流.以上结果表明成年Wistar大鼠的海马HPCs可表达功能性的L-型钙通道.
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| 关 键 词： | 成年大鼠 海马 神经前体细胞 L-型钙通道 |
| 收稿时间： | 2005-04-11 |
| 修稿时间： | 2005-04-112005-04-30 |
Hippocampal Neural Progenitor Cells Express Functional L-Type Calcium Channel in Adult Rat |
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| FENG Jin-Li,HU De-Hui,CHEN Ming,TIAN Ying-Hong,SHI Zhi-Min,YANG Jian-Ming,ZHU Xin-Hong,LI Xiao-Wen and GAO Tian-Ming. Hippocampal Neural Progenitor Cells Express Functional L-Type Calcium Channel in Adult Rat[J]. Progress In Biochemistry and Biophysics, 2005, 32(9): 822-828 |
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| Authors: | FENG Jin-Li HU De-Hui CHEN Ming TIAN Ying-Hong SHI Zhi-Min YANG Jian-Ming ZHU Xin-Hong LI Xiao-Wen GAO Tian-Ming |
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| Affiliation: | Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China;Department of Anatomy, Department of Physiology, The First Military Medical University, Guangzhou 510515, China |
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| Abstract: | |
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| Keywords: | adult rat hippocampus neural progenitor cells L-type calcium channel |
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