The targeted disruption of the CD98 gene results in embryonic lethality |
| |
Authors: | Tsumura Hideki Suzuki Noboru Saito Hiromitsu Kawano Mitsuo Otake Seiichi Kozuka Yuji Komada Hiroshi Tsurudome Masato Ito Yasuhiko |
| |
Affiliation: | Functional Genomics Institutes, Life Science Research Center, Mie University, 2-174, Edobashi, Tsu-Shi, Mie Prefecture 514-8507, Japan. |
| |
Abstract: | CD98 is one of the important molecules for development, cell differentiation, cell proliferation, and regulation of cellular function. In this study, CD98 heavy chain (HC) knockout mice were produced and analyzed. Five targeted ES clones were obtained and colony frequency was about 2%. One (clone 113) of the five heterozygous ES cell clones had undergone aberrant recombination at the 5' side. The aberrant recombination happened at the site between second intron and 5' arm. All lines from correctly targeted clones could not transmit the mutated allele to spermatozoa. The mutated allele derived from the aberrant targeted clone was transmitted to the progeny. However, none of the F2 mice was homozygous for the CD98 mutation, indicating that the targeted disruption of the CD98 gene results in embryonic lethality. The point of embryonic lethality is considered to be between 3.5 and 9.5 dps. These findings indicate that CD98 molecules are essential for mouse embryogenesis. |
| |
Keywords: | CD98 FRP-1 Embryonic lethality Knockout mouse |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|