Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro |
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Authors: | Kateryna Kotsarenko Valentyna Lylo Tetiana Ruban Larysa Macewicz Lyubov Lukash |
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Affiliation: | 1.University of South Bohemia in ?eské Budějovice,Ceske Budejovice,Czech Republic;2.Institute of Molecular Biology and Genetics of NAS of Ukraine,Kiev,Ukraine |
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Abstract: | The inducible repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates O6-methylguanine adducts in DNA and protects the cells from damaging effects of alkylating agents. We have found that anti-MGMT antibodies recognize both the MGMT protein with a mol. weight?~?24 kDa and a protein with a mol. weight?~?48 kDa, which was named MARP (anti-methyltransferase antibody recognizable protein). A number of growth factors and cytokines were shown to regulate the expression of MGMT and MARP proteins. The ranges of concentrations of several growth factors and cytokines that caused increasing or decreasing protein amounts in human cell cultures were determined. The results of special biological experiments have allowed us to assume a possible role of MARP in the repair of alkyl adducts in human cells. |
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