Genome Editing of Plants |
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| Authors: | D. D. Songstad J. F. Petolino D. F. Voytas N. A. Reichert |
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| Affiliation: | 1. Cibus LLC, San Diego, California, USA;2. Biotechnology Department, Ivy Tech Community College, Indianapolis, Indiana, USA;3. Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, Minnesota, USA;4. Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi, USA |
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| Abstract: | Genome editing in organisms via random mutagenesis is a naturally occurring phenomenon. As a technology, genome editing has evolved from the use of chemical and physical mutagenic agents capable of altering DNA sequences to biological tools such as designed sequence-specific nucleases (SSN) to produce knock-out (KO) or knock-in (KI) edits and Oligonucleotide Directed Mutagenesis (ODM) where specific nucleotide changes are made in a directed manner resulting in custom single nucleotide polymorphisms (SNPs). Cibus' SU Canola?, which the US Department of Agriculture (USDA) views as non-genetically modified (non-GM), is Cibus' first commercial product arising from plant genome editing and had its test launch in 2014. Regulatory aspects of the various genome editing tools will be discussed. |
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| Keywords: | Biological mutagenesis chemical mutagenesis CRISPR knock-in knock-out ODM RTDS single nucleotide polymorphism SNP TALENs ZFN |
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