Effect of plant growth regulators on growth and biosynthesis of phenolic compounds in genetically transformed hairy roots of<Emphasis Type="Italic">Panax ginseng</Emphasis> C. A. Meyer |
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Authors: | Gwi-Taek Jeong Je-Chang Woo " target="_blank">Don-Hee Park |
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Institution: | 1.School of Biological Sciences and Technology,Chonnam National University,Gwangiu,Korea;2.Faculty of Applied Chemical Engineering,Chonnam National University,Gwangju,Korea;3.Biotechnology Research Institute,Chonnam National University,Gwangju,Korea;4.Institute of Bioindustrial Technology,Chonnam National University,Gwangju,Korea;5.Research Institute for Catalysis,Chonnam National University,Gwangju,Korea;6.Engineering Research Institute,Chonnam National University,Gwangju,Korea;7.Department of Biology,Mokpo National University,Jeonnam,Korea |
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Abstract: | In this study, morphological alterations, biomass growth, and secondary metabolite production of genetically transformed hairy
roots ofPanax ginseng C. A. Meyer, were evaluated after administration of plant growth regulators. The addition of benzylamino purine and kinetin
to the culture media increased biomass formation and phenolic compound biosynthesis in the hairy roots. α-Naphthaleneacetic
acid and indole-3-butyric acid inhibited hairy root growth, however, low concentrations of indole-3-acetic acid slightly increased
hairy root growth. Low concentrations of 2,4-Dichlorophenoxyacetic acid profoundly inhibited growth of hairy roots. The addition
of plant growth regulators, such as auxin, did not increase total phenolic compounds in hairy roots that did not contain gibberellic
acid and cytokinins. Callus formation was induced in cultures suspended in liquid medium amended with benzylamino purine and
kinetin. Hairy roots regenerated from these calluses exhibited an active growth pattern with extensive lateral branching in
non-amended medium, similar to the growth pattern of the original hairy roots. |
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Keywords: | Panax ginseng transformed hairy root plant growth regulator phenolic compounds |
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