Induction of 2,3-bisphosphoglycerate synthase in Friend leukemia cells

Friend leukemia cells (clone 745A) induced to differentiate with dimethylsulfoxide showed at least a 10-fold increase of 2,3-bisphosphoglycerate synthase and concomitant accumulation of 2,3-bisphosphoglycerate. These changes paralelled that of the number of hemoglobin-positive cells. Accumulation of 2,3-bisphosphoglycerate was also induced by dimethylsulfoxide in the other clone C-10-6, but not in C-9-6 which is resistant to differentiation with dimethylsulfoxide. Induced activity of 2,3-bisphosphoglycerate synthase in clone 745A was neutralized by antiserum prepared from a rabbit which was immunized with human erythrocyte 2,30bisphosphoglycerate synthase. By using this antiserum, biosynthesis of 2,3-bisphosphoglycerate synthase was detected in Friend cells only after induction by dimethylsulfoxide.