Optimization of a liquid medium for beauvericin production in <Emphasis Type="Italic">Fusarium redolens</Emphasis> Dzf2 mycelial culture |
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Authors: | Li-Jian Xu Yuan-Shuai Liu Li-Gang Zhou Jian-Yong Wu |
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Institution: | (1) Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong;(2) Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China;(3) College of Agricultural Resource and Environment, Heilongjiang University, 15000 Harbin, China; |
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Abstract: | Beauvericin (BEA) is a proven and potent antibiotic compound useful for bio-control and a potential antifungal and anticancer
agent for human. This study was to evaluate and optimize the nutrient medium for BEA production in mycelial liquid culture
of a high BEA-producing fungus Fusarium redolens Dzf2 isolated from a medicinal plant. Among various organic and inorganic carbon and nitrogen sources, glucose and peptone
were found the most favorable for the F. redolens Dzf2 mycelial growth and BEA production. Through a Plackett-Burman screening test on a basal medium, glucose, peptone, and
medium pH were identified as the significant factors for mycelial growth and BEA production. These factors were optimized
through central composite design of experiments and response surface methodology, as 49.0 g/L glucose, 13.0 g/L peptone and
pH 6.6, yielding 198 mg/L BEA (versus 156 mg/L in the basal medium). The BEA yield was further increased to 234 mg/L by feeding
10 g/L glucose to the culture during exponential phase. The results show that F. redolens Dzf2 mycelial fermentation is a feasible and promising process for production of BEA. |
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