DNA-binding domains of fibronectin probed using Western blots

Human plasma fibronectin was subjected to limited proteolysis, and its DNA-reactive polypeptides were distinguished using the protein blot technique. The polypeptides were separated electrophoretically by SDS gel electrophoresis, transferred to nitrocellulose and probed with radiolabeled human lymphocyte DNA. The major DNA-binding domains identified by the protein blots were comparable to the polypeptides identified using affinity chromatography on DNA-cellulose.