Heparin-released triglyceride lipase from chang liver cells |
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Authors: | Fumio Ogata Yoshihei Hirasawa |
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Institution: | Research Division, Shinrakuen Hospital, Nishiariake-cho 1-27, Niigata-shi, 950-21, Japan |
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Abstract: | Heparin-released triglyceride lipase (TGL) from Chang liver cells (ATCC CCL 13) was investigated using very low density lipoproteins (VLDL) as a substrate. The TGL activity was released into the culture medium when the cells were incubated with heparin. The enzyme showed maximum activity at pH 8.5, and 80% inhibition by 0.6 M NaCl. These results indicated that Chang liver cells, a cell line derived from liver, synthesize lipoprotein lipase. |
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