Binding of 4-methylumbelliferyl-galactosides to peanut agglutinin |
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Authors: | Monique Decastel Anh-Tuan Tran Jean-Pierre Frénoy |
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Institution: | Biologie et Pathologie Moléculaires des Glycoprotéines, C.N.R.S. L.A. 293, U.E.R. Biomédicale des Saints-Pères, 75006 Paris, France. |
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Abstract: | The binding of 4-methylumbelliferyl-α-D-galactopyranoside, -β-D-galactopyranoside and -D-Galβ(1→3)DGalNac to peanut agglutinin was studied by fluorescence. Peanut agglutinin quenched the fluorescence intensity of 4-methylumbelliferyl-α-D-galactopyranoside but enhanced that of the two 4-methylumbelliferyl-β-galactosides. For α-D-galactopyranoside, the association constants measured at 4 and 25°C were 3.4 × 103 and 1.7 × 103 M?1 respectively, and for D-Galβ(1→3)DGalNac, 1.5 × 105 and 3.3 × 104 M?1. The binding enthalpies estimated from these values are consistent with the existence of extended sugar binding sites in the peanut agglutinin molecule. |
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