The lipoxygenase product, 5-hydroperoxy-arachidonic acid,augments chemotactic peptide-stimulated arachidonic acid release from HL60 granulocytes

The chemotactic peptide N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys stimulates the release of arachidonic acid from endogenous phospholipids of the human promyelocytic leukemia cell line HL60. This release of unsaturated fatty acid is augmented by the presence of exogenously added lipoxygenase product, 5-hydroperoxy-arachidonic acid (5-HPETE). Other hydroperoxy- and hydroxy-arachidonic acid derivatives are less potent. In addition, saturated fatty acids and hydrogen peroxide do not possess this ability to augment arachidonic acid release. None of the arachidonic acid derivatives are capable of stimulating the release of arachidonic acid from endogenous phospholipids in the absence of the chemotactic peptide.