Influence of the N- and C-terminal tails on the structure of the globular head of histone H1

The influence of the N- and C-terminal tails with respect to the conformation of the H1 central domain was investigated by studying the changes of both dichroism circular and absorption spectra during the course of limited tryptic digestion of histones H1 from calf thymus and from the fruit fly Ceratitiscapitata. The removal of the terminal tails of histone H1 results in a conformational change of the globular domain and the results suggest that the mutual interactions of the more charged N- and C-terminal regions with the central region of H1 modulate the precise structure of the globular head.