Decreased [3H]serotonin and [3H]spiperone binding consequent to lipid peroxidation in rat cortical membranes |
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Authors: | S.F. Muakkassah-Kelly J.W. Andresen J.C. Shih P. Hochstein |
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Affiliation: | 1. Institute for Toxicology, School of Pharmacy University of Southern California Los Angeles, California 90033 USA;2. Department of Biochemistry, School of Medicine University of Southern California Los Angeles, California 90033 USA |
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Abstract: | The effect of lipid peroxidation on two types of serotonin binding sites was investigated. Incubation of rat cortical membranes with an ascobate-dependent peroxidizing system resulted in the formation of malonyldialdehyde and significant decreases in the specific binding of [3H]serotonin and [3H]spiperone to the treated membranes. When ascorbate concentrations were varied from 0.025 to 6.0 mM, malonyldialdehyde production increased to a maximum at 0.5 mM ascorbate and then declined. Conversely, the specific binding of [3H]serotonin and [3H]spiperone decreased to a minimum at 0.5 mM ascorbate and then increased. Regression analysis of the data revealed that the decrease in the two binding sites was linearly correlated to lipid peroxidation. |
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