Activation of tubulinyl-tyrosine carboxypeptidase by spermine,spermidine and putrescine |
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Authors: | Héctor S Barra Carlos E Argaran?a |
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Institution: | Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000-Córdoba, R. Argentina |
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Abstract: | Spermine, spermidine and putrescine produce dose dependent stimulation of the invitro tubulinyl-tyrosine carboxypeptidase. Maximal stimulation was obtained with spermine, spermidine or putrescine at 0.06 mM, 1 mM and 6 mM, respectively. At higher concentrations, the enzyme activity was inhibited. The enzyme was also activated by Mg++; the concentration formaximal effect was 4–6 mM. The stimulation produced by optimal concentration of each amine was unaffected by Mg++ up to 2 mM; higher concentration of Mg++ showed inhibitory effect. At optimal Mg++ concentration, the carboxypeptidase activity was inhibited by increasing amine concentration. The amines at 0.5 or 5 mM did not produce any effect on the incorporation of tyrosine catalyzed by tubulin tyrosine ligase. |
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Keywords: | TTL tubulin tyrosine ligase TTCPase tubulinyl-tyrosine carboxypeptidase |
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