Differential induction of rat hepatic cytochrome P-448 and glutathione S-transferase B messenger RNAS by 3-methylcholanthrene |
| |
| Authors: | Cecil B Pickett Claudia A Telakowski-Hopkins Ann Marie Donohue Anthony YH Lu Barbara F Hales |
| |
| Institution: | 1. Department of Biochemical Regulation Merck Sharp & Dohme Research Laboratories Rahway, New Jersey 07065 USA;2. Department of Animal Drug Metabolism, Merck Sharp & Dohme Research Laboratories Rahway, New Jersey 07065 USA |
| |
| Abstract: | Liver poly(A+)-RNA isolated from untreated and 3-methylcholanthrene treated rats has been translated in the rabbit reticulocyte cell-free system in order to determine the level of translationally active cytochrome P-448, glutathione S-transferase B and serum albumin mRNAs. Translatable cytochrome P-448 mRNA was not detected in untreated rats; however in animals treated with 3-methylcholanthrene cytochrome P-448 mRNA was elevated markedly. Functional rat liver glutathione S-transferase B mRNA was elevated 2-fold by 3-methylcholanthrene administration, whereas the serum albumin mRNA level was decreased by 50%. Our results indicate that 3-methylcholanthrene is not just a specific inducer of drug metabolizing enzymes but can alter the mRNA level encoding other polypeptides and thus affect cellular homeostasis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
