Preliminary characterization of chelation-sensitive nucleoprotein particles |
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| Authors: | David A Beary Douglas L Vizard Ronald A LaBiche Kenneth J Hardy Sara E Bryan |
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| Institution: | 1. Department of Biological Sciences, University of New Orleans New Orleans, LA 70148 USA;2. Department of Physics, The University of Texas System Cancer Center M.D. Anderson Hospital and Tumor Institute Houston, TX 77030 USA |
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| Abstract: | Nucleoprotein particles (B2), isolated following digestion of calf thymus chromatin with micrococcal nuclease, are resolved on a non-chelating Bio-Gel A-5m column. B2 protein electrophoresis showed the presence of several H1 species and several nonhistone proteins but was depleted in core histones. DNA electrophoresis demonstrated that native B2 DNA has a length of about 46 base pairs. On DNA sequencing gels, the length distribution of denatured B2 DNA ranged from 12 to 35 bases with a weighted average chain length of about 26 bases. Depletion of a 20 base band in B2 DNA suggested specific protection of internucleosomal DNA sites during the nuclease digestion. |
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| Keywords: | Author to whom all correspondence should be sent |
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