Activation of the plastid isozyme of phosphofructokinase from developing endosperm of Ricinuscommunis by fructose 2,6-bisphosphate |
| |
Authors: | Jan A Miernyk David T Dennis |
| |
Institution: | Biology Department, Queen''s University, Kingston, Ontario, Canada K7L 3N6 |
| |
Abstract: | The plastid isozyme of phosphofructokinase from developing castor oil seeds is stimulated by low concentrations of fructose 2,6-bisphosphate when assayed at pH 7.0. The stimulation involves a shift in fructose 6-phosphate kinetics from sigmoidal to near hyperbolic. The plastid isozyme is unaffected by fructose 2,6-bisphosphate when assayed at pH 8.0, and the cytosolic isozyme is unaffected at either pH 7.0 or 8.0. There is no interaction between fructose 2,6-bisphosphate and the other regulators of the phosphofructokinases; phosphoenolpyruvate, 2-phosphoglycerate, 3-phosphoglycerate and inorganic phosphate. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|