Specific and saturable binding of albumin to rat adipocytes: Modulation by epinephrine and possible role in free fatty acid transfer |
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Authors: | Ruth Brandes Robert K Ockner Richard A Weisiger Nina Lysenko |
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Institution: | 1. Department of Medicine, University of California San Francisco, California 94143 USA;2. Liver Center, University of California San Francisco, California 94143 USA |
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Abstract: | Specific and saturable binding of 125I-bovine albumin to rat adipocytes in suspension was observed (apparent Kd 2.09 ± 0.52 × 10?6 M; 8.58 ± 2.49 × 106 sites per cell; mean ± SEM). The binding was rapid and reversible for at least 10 min, suggesting that endocytosis of albumin was minor under assay conditions. Pre-incubation of cells with epinephrine bitartrate caused an apparent increase in number and decrease in affinity of the adipocyte binding sites for albumin. These findings suggest that a specific and saturable interaction of albumin with the adipocyte surface may play a role in the cellular uptake and release of free fatty acids. |
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Keywords: | To whom requests for reprints should be adressed |
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