Suicidal inactivation of microsomal cytochrome P-450 by halothane under hypoxic conditions |
| |
Authors: | Herbert de Groot Uwe Harnisch Thomas Noll |
| |
Institution: | Institut für Physiologische Chemie I der Universität Düsseldorf Moorenstraße 5, D-4000 Düsseldorf, West Germany |
| |
Abstract: | Under anaerobic conditions the addition of halothane to NADPH-reduced liver microsomes from phenobarbital-pretreated male rats resulted in a pronounced inactivation of microsomal cytochrome P-450, presumably produced by covalent binding of reactive halothane metabolites such as the CF3CHCl-radical. Compared with microsomes from phenobarbital-pretreated rats, the loss of active cytochrome P-450 was markedly decreased in microsomes from both 3-methylcholanthrene-pretreated and untreated rats. Increasing the O2-partial pressure decreased the amount of cytochrome P-450 inactivated by halothane metabolites. At an O2-partial pressure of approximately 40 mm Hg the inactivation was virtually eliminated. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |