Comparison of yeast mitochondrial Phe-tRNA synthetase subunits to their cytoplasmic counterparts: Isolation and determination of amino acid compositions |
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| Authors: | Martin Diatewa Yves Boulanger AndréJC Stahl |
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| Institution: | 1. Laboratoire de Biochimie, Faculté de Pharmacie, Université Louis Pasteur, B.P. 10, 67048 Strasbourg Cedex, France;2. Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., 15, rue Descartes, 67000 Strasbourg, France. |
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| Abstract: | The α and β subunits of yeast mitochondrial Phe-tRNA synthetase are separated and isolated by means of chromatography on DEAE-cellulose, after enzyme alkylation with iodoacetate. The comparison of amino acid compositions of yeast mitochondrial and cytoplasmic native Phe-tRNA synthetases and their components shows significant differences. Results indicate that the two enzymes are coded for by different nuclear genes. |
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| Keywords: | mit mitochondrial ct cytoplasmic PheRS phenylalanyl-tRNA synthetase |
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