Kirromycin-resistant elongation factor Tu from pulvomycin-producing wild-type of streptoverticillium mobaraense |
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Authors: | Claudia Glöckner Walter Wörner Heinz Wolf |
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Affiliation: | Institut für Biologie II, Lehrstuhl für Mikrobiologie I der Universität, D-7400 Tübingen, Federal Republic of Germany |
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Abstract: | Elongation factor Tu (EF-Tu) ofStreptoverticillium mobaraense, which produces pulvomycin, has been prepared to 90% purity. The purified protein differs significantly from the analogous protein found inEscherichia coli in molecular weight and antibiotic sensitivity. EF-Tu migrates in sodium dodecyl sulfate gel electrophoresis as a 46,000-dalton species. The protein is sensitive to pulvomycin, but highly resistant to kirromycin. EF-Tu fromStv. mobaraense exists in multiple forms as monomer and polymers. By contrast to the monomer, the polymers of EF-Tu are completely resistant to pulvomycin. |
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Keywords: | EF-Tu elongation factor Tu EF-G elongation factor G SDS sodium dodecyl sulfate |
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