Endogenous phospholipase and choline release in human erythrocytes: A study using 1H NMR spectroscopy |
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| Authors: | Bogdan E Chapman Georg R Beilharz Michael J York Philip W Kuchel |
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| Institution: | Department of Biochemistry, University of Sydney, Sydney, NSW, 2006, Australia |
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| Abstract: | 1H spin-echo NMR spectroscopy has been used to show for the first time, to our knowledge, the presence of a phospholipase D activity in human erythrocytes. The enzyme is presumably intra-cellular and we have demonstrated that it is activated by a component of Krebs bicarbonate medium; most probably calcium ions. The existence of a phospholipase in human erythrocytes allows a simple explanation for the choline accumulation that occurs in these cells of manic-depressive and other patients receiving lithium carbonate therapy. |
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