Substrate-dependent inhibition of yeast enolase by fluoride |
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Authors: | S.G. Spencer J.M. Brewer |
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Affiliation: | Department of Biochemistry University of Georgia Athens, Georgia 30602 USA |
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Abstract: | A possibly physiologically significant inhibition of yeast enolase by fluoride occurs in the absence of inorganic phosphate. The inhibition increases with time, is strongly dependent on fluoride concentration and requires substrate and “catalytic” Mg2+. The inhibition increases more slowly in the presence of product (phosphoenolpyruvate) than substrate (2-phosphoglycerate). The dependence on fluoride concentration and the spans of substrate analogue displacement titrations suggest the inhibition is produced by two moles of fluoride per active site. |
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Keywords: | EDTA Ethylenediamine tetraacetic acid DMSO Dimethyl sulfoxide |
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