Somatic embryogenesis from immature embryos in meadowfoam (Limnanthes alba) |
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Authors: | Darlene Southworth Stephen Kwiatkowski |
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Affiliation: | (1) Department of Biology, Southern Oregon State College, 97520 Ashland, OR, USA |
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Abstract: | Developing embryos from immature seeds were excised and cultured. Optimal proliferation of differentiated secondary embryos occurred on Murashige-Skoog media containing 7% sucrose, 0.1 M 2,4-D, and 0.1–1.0 M zeatin. Higher levels of auxins inhibited embryo proliferation. Secondary embryos were subcultured to produce more embryos. The results indicate the feasibility of clonal propagation of meadowfoam.Abbreviations 2,4-D 2,4-dichlorophenoxyacetic acid - BA 6-Benzyladenine |
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Keywords: | Limnanthes alba meadowfoam somatic embryogenesis tissue culture |
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