An improved system for controlled rate cooling of biological material |
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| Authors: | K Kalmbach M R Mardiney |
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| Affiliation: | 1. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;3. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4. Department of Ocean and Earth Sciences, Old Dominion University, 4600 Elkhorn Avenue, Norfolk, Virginia 23529, USA |
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| Abstract: | The unit in present operation at the Baltimore Cancer Research Center has afforded far greater accuracy than freezing control units now commercially available. Its versatility in allowing direct dialing of the cooling rate and feedback control from the sample phantom allows for a completely automated cooling system which will take the specimen smoothly through the heat of fusion while maintaining a constant predetermined slope. An example of a typical freezing curve depicting lymphocytes frozen in 1640 media containing 10% DMSO as previously described (1–3) is shown in Fig. 2.The present unit is wired to function with the Linde BF4-1 and BF4-2 freezing chambers; however, any similar chamber could be used by providing the proper electrical connections and sample sensor.The cost of the controllers component parts will vary according to the number of features desired but for the unit described here it is approximately $350.00. |
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