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Cell structure and mobilization of lipids and proteins from cotyledon under microgravity influence
Authors:Olena Nedukha  Elizabeth Kordyum  Christopher Brown
Institution:Institute of Botany, Tereschenkivska str., Kiev, Ukraine. cell@svitonline.com
Abstract:The structural-functional organization of cotyledon parenchyma cells of 6-day soybean (Glycine max L.) seedlings that were grown on board the space Shuttle Columbia (STS-87) have been studied. The purafil (KMnO4) was used in the experiment for the removing of some part of ethylene that secretes out from seedlings. There were four variants of the experiment: ground control (+purafil), ground control (-purafil), microgravity (+purafil) and microgravity (-purafil). The electron microscopy, srereological, and pyroantimonate cytochemical methods have been used. It is established the some indices of changes of storage substances in cotyledon parenchyma cells under influence of microgravity. It is displayed in the change of cell ultrastructure, the decrease of relative volume of storage cytoplasmic lipid bodies, a disappearance of storage protein body into vacuole and the redistribution of ionized calcium in cell. It was supposed that microgravity is influenced on the acceleration of storage substances catabolism.
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