Distribution of sarcoplasmic reticulum Ca-ATPase and of calsequestrin at the polar regions of rat,rabbit and cat intrafusal fibers |
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| Authors: | A Maier E Leberer D Pette |
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| Institution: | (1) Department of Cell Biology and Anatomy, University of Alabama at Birmingham, 35294 Birmingham, AL, USA;(2) Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750 Konstanz, Federal Republic of Germany;(3) Present address: Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, 112 College Street, M5G 1L6 Toronto, Ontario, Canada |
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| Abstract: | Summary Sarcoplasmic reticulum (SR) Ca2+-pumping ATPase (Ca-ATPase) and calsequestrin (CaS) were visualized by indirect immunofluorescence at the polar regions of adult rat, rabbit and cat intrafusal fibers. The immunohistochemical reaction products were regarded as histochemical markers of the SR and as valid indicators of the distribution of the two Ca2+sequestering proteins. Static nuclear bag2 fibers displayed lower levels of both Ca-ATPase and CaS than the other two intrafusal fiber types. Nuclear chain fibers presented the highest Ca-ATPase levels and, together with dynamic nuclear bag1 fibers, they also exhibited relatively high amounts of CaS. The level of Ca-ATPase was lower in bag1 fibers than in nuclear chain fibers, but not as low as in bag2 fibers. The comparatively high levels of Ca-ATPase and CaS seen in nuclear chain fibers coincided with their reported faster contractile speeds compared to nuclear bag fibers.Dedicated to Professor Dr. T.H.Schiebler on the occasion of his 65th birthday |
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