Efficient double-strand scission of plasmid DNA by quaternized-chitosan zinc complex |
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| Authors: | Zhang Qi Xiang Yan Liang Dawei Peng Yinggui Guo Hong |
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| Affiliation: | School of Materials Science and Engineering, Beihang University, Haidian District, Beijing, China. |
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| Abstract: | N-[(2-Hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTACC) was prepared to construct a chitosan-based zinc complex (HTACC-Zn(II)) as a catalyst with good water solubility for rapid DNA cleavage. Results indicated that the observed rate constant (k(obs)) of plasmid DNA cleaved by HTACC-Zn(II) could be enhanced by 10(7)-fold compared with that of uncatalyzed DNA cleavage. The kinetic behavior of HTACC-Zn(II) for DNA cleavage is well fitted by Michaelis-Menten model. The results of gel electrophoresis suggested that HTACC-Zn(II) preferentially perform double-strand break of plasmid DNA. |
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