Guinea pig serum l-asparaginase: Purification, and immunological relationship to liver l-asparaginase and serum l-asparaginases in other mammals |
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Authors: | N. Zhang F. Clarke G. Di Trapani D. Keough I. Beacham |
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Abstract: | l-asparaginase, an enzyme used in the treatment of acute lymphocytic leukemia, is found in the serum of only a few mammalian groups, including the guinea pig and its close relatives in the superfamily Cavioidea. This report describes the purification and characterization of l-asparaginase from guinea pig serum. Antiserum against the purified enzyme cross-reacted with sera from other Cavioidean species but not with mouse serum. Relatively weak cross-reaction with unpurified l-asparaginase in guinea pig liver indicates a significant degree of evolutionary divergence. |
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Keywords: | Guinea pig l-Asparaginase Serum Immunoblotting Isozymes Cavioidea Dye-ligand chromatography Preparative gel electrophoresis |
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