Total Uptake and Incorporation into DNA of 3H-Thymidine, 3H-Deoxyuridine, and 3H-Thymine in the Primary Root of Vicia faba L.: II. EXCISED ROOTS |
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Authors: | MACLEOD R D |
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Abstract: | Total uptake of 3H-thymidine, 3H-thymine, and 3H-deoxyuridineand incorporation of these substances into DNA have been investigatedin excised roots in Vicia faba. Total uptake was found to behigher in excised roots than in intact ones. This was a consequenceof exogenous 3H-DNA precursors entering the excised roots throughthe cut surface. Where the cut surface was not immersed in the3H-thymidine solution 3H uptake was also higher than in intactroots. In this case 3H uptake scemed to be correlated with water-lossfrom the cut surface. Incorporation of 3H-thymidine, 3H-thymine,and 3H-deoxyuridine into DNA was found to be higher in excisedroots than in intact ones, probably as a result of a decreasein the size of the relevant endogenous precursor pools. It issuggested that this decrease resulted from the cessation ofthe supply of DNA precursors to these roots from the cotyledons. |
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