Distribution of sodium transporters and aquaporin-1 in the human choroid plexus |
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Authors: | Praetorius Jeppe Nielsen Søren |
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Institution: | The Water and Salt Research Center, Institute of Anatomy, Univ. of Aarhus, Wilhelm Meyers Allé, Bldg. 234, 8000 Aarhus C, Denmark. jp@ana.au.dk |
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Abstract: | The choroid plexus epithelium secretes electrolytes and fluid in the brain ventricular lumen at high rates. Several channels and ion carriers have been identified as likely mediators of this transport in rodent choroid plexus. This study aimed to map several of these proteins to the human choroid plexus. Immunoperoxidase-histochemistry was employed to determine the cellular and subcellular localization of the proteins. The water channel, aquaporin (AQP) 1, was predominantly situated in the apical plasma membrane domain, although distinct basolateral and endothelial immunoreactivity was also observed. The Na+-K+-ATPase 1-subunit was exclusively localized apically in the human choroid plexus epithelial cells. Immunoreactivity for the Na+-K+-2Cl cotransporter, NKCC1, was likewise confined to the apical plasma membrane domain of the epithelium. The Cl/HCO3 exchanger, AE2, was localized basolaterally, as was the Na+-dependent Cl/HCO3 exchanger, NCBE, and the electroneutral Na+-HCO3 cotransporter, NBCn1. No immunoreactivity was found toward the Na+-dependent acid/base transporters NHE1 or NBCe2. Hence, the human choroid plexus epithelium displays an almost identical distribution pattern of water channels and Na+ transporters as the rat and mouse choroid plexus. This general cross species pattern suggests central roles for these transporters in choroid plexus functions such as cerebrospinal fluid production. immunohistochemistry; metabolism; cerebrospinal fluid secretion |
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