Effect of growth rate on ethanol tolerance ofSaccharomyces cerevisiae |
| |
Authors: | ? Novotný M Flieger J Pano? L Dole?alová |
| |
Institution: | (1) Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
| |
Abstract: | Δ5,7
Saccharomyces cerevisiae cells growing in chemostat at a specific growth rate of 0.075/h exhibited higher ethanol tolerance measured as ethanol-induced
death and anaerobic growth inhibition than the cells growing at 0.2/h, the difference being dependent on the carbon-to-nitrogen
molar proportion in the medium. The observed difference in sensitivity to ethanol of anaerobic growth between the slowly and
rapidly-growing cells was completely reversed as a result of a block in sterol synthesis causing a negligible synthesis of
Δ5,7. Two physiological parameters, budding frequency and membrane composition, evidently affected ethanol tolerance. Differences
between the Δ5,7 and deficient strains documented a profound effect of the quality of the sterol present on the physiological state of the
cell. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|