| 一种提取野生苎麻总DNA的方法 |
| |
| 引用本文： | 黄小英,刘瑛,赖小萍,彭学渊,赖占钧. 一种提取野生苎麻总DNA的方法[J]. 中国野生植物资源, 2002, 21(1): 56-57 |
| |
| 作者姓名： | 黄小英 刘瑛 赖小萍 彭学渊 赖占钧 |
| |
| 作者单位： | 江西省苎麻生物技术重点实验室,宜春,336000 |
| |
| 摘 要： | 用CTAB法提取野生苎麻总DNA ,结合野生苎麻中酚类、黄酮类等次生代谢物质多的特点 ,加入一定浓度的PVP、β—巯基乙醇 ,可以获得高分子量DNA ,经实验鉴定 ,其纯度符合分子遗传实验要求。
|
| 关 键 词： | 野生苎麻 CTAB法 总DNA |
A Method for Extracting Total DNA of Wild Ramie |
| |
| Huang Xiaoying,Liu Ying,Lai Xiaoping,Peng Xueyang,Lai Zhanjun. A Method for Extracting Total DNA of Wild Ramie[J]. Chinese Wild Plant Resources, 2002, 21(1): 56-57 |
| |
| Authors: | Huang Xiaoying Liu Ying Lai Xiaoping Peng Xueyang Lai Zhanjun |
| |
| Abstract: | Through the method of CTAB,Total DNA of wild Ramie was extracted.According to the character of wild Ramie that it contains lots of secondary metabolites such as alkaloid and phenli, high molecular mass DNA can be obtained by adding certain concentration of PVP,Mercapto Ethanol into extraction buffer. The results of the experiment show its purity can satisfy the demand of molecular genetic experiment. |
| |
| Keywords: | Wild Ramie CTAB method Total DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
