| Abstract: | A critical nutrient for the growth of SV3T3 cells is iron. Iron must be added in the ferrous form or, if in the ferric state, with a suitable complexing agent. Both transferrin and hemoglobin, as iron complexes, will stimulate cell growth in biotin supplemented medium either with low serum (0.15% v/v) or serum-free. The growth stimulation by iron (free or in complexed form) is dependent on the presence of biotin in the medium. These results indicate the importance of transferrin as a serum growth factor. |
