Biotransformations by microbial Baeyer-Villiger monooxygenases stereoselective lactone formationin vitro by coupled enzyme systems |
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| Authors: | Gideon Grogan Stanley Roberts Andrew Willetts |
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| Institution: | (1) Department of Biological Sciences, University of Exeter, EX4 4QG Exeter, Devon, England;(2) Department of Chemistry, University of Exeter, EX4 4QG Exeter, Devon, England |
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| Abstract: | Summary The regio- and stereoselective biotransformation of bicyclo (3. 2. 0) hept-2-en-6-one by the NADH-dependent Baeyer-Villiger monooxygenase from camphorgrownPseudomonas putida NCIMB 10007 has been shown to yield a chiral lactone not accessible by curently-used biocatalysts. The biotransformation can be conductedin vitro using two alternative coupled enzyme systems (alcohol dehydrogenase and monooxygenase: formate dehydrogenase and monooxygenase) within situ recycling of NAD+/NADH. |
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