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产金属硫蛋白酿酒酵母诱变菌株的生物学功能研究
引用本文:尤兰兰,周波,姚良同,贾乐. 产金属硫蛋白酿酒酵母诱变菌株的生物学功能研究[J]. 微生物学杂志, 2005, 25(1): 36-39
作者姓名:尤兰兰  周波  姚良同  贾乐
作者单位:山东农业大学,生命科学学院,山东,泰安,271018
基金项目:山东省教委资助项目 (3 2 174)
摘    要:以酿酒酵母 (Saccharomycescerevisiae)单倍体菌株SY1为出发菌株 ,通过紫外线和亚硝基胍复合诱变 ,获得 1株Cu2 高抗性突变株 ,并对其生物学功能进行了研究。结果表明 ,其拮抗紫外线 ,拮抗电离辐射 ,清除·OH自由基能力 ,Cu2 解毒能力均比出发菌株有所提高 ,经 5 0世代培养后 ,其遗传稳定性保持在 96 %以上。

关 键 词:酿酒酵母  金属硫蛋白  诱变菌株  生物学功能
文章编号:1005-7021(2005)01-0036-04
修稿时间:2003-11-07

Biological Function of Metallothionein Producing Saccharomyces cerevisiae Mutant
YOU Lan-lan,ZHOU Bo,YAO Liang-tong,Jia Le. Biological Function of Metallothionein Producing Saccharomyces cerevisiae Mutant[J]. Journal of Microbiology, 2005, 25(1): 36-39
Authors:YOU Lan-lan  ZHOU Bo  YAO Liang-tong  Jia Le
Affiliation:Coll of Life Sci. Shandong Agric. Univ. Tai'an 271018
Abstract:A high Cu{2} resistant Saccharomyces cerevisiae (SC) mutant was obtained by composite mutation with UV and NTG and SC monoploid as starting strain. Later its biological function was studied. The results showed that its resistance against UV and ionizing radiation, OH free radical clearing, and detoxification against Cu{2} were higher to some extent than the starting strain, and its genetic stability was maintained over 96%.
Keywords:Saccharomyces cerevisiae  metallothionein  mutant  biological functioni
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