Flow sorting and culture of protoplasts: Conditions for high-frequency recovery,growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana |
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Authors: | D W Galbraith C L Afonso K R Harkins |
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Institution: | (1) School of Biological Sciences, University of Nebraska-Lincoln, 68588-0118 Lincoln, Nebraska, USA |
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Abstract: | Protoplasts were prepared from suspension cultures of Nicotiana tabacum cv Wisconsin 38 that had been prelabeled with FITC. The protoplasts were subjected to flow sorting based on fluorescence content using a Coulter EPICS V Flow Cytometer — Cell Sorter. Conditions were established that allowed the recovery after sorting of approximately 30% of the initial protoplasts in a viable state. These were subsequently regenerated into calli that underwent shoot morphogenesis.Abbreviations FITC
Fluorescein isothiocyanate
- FDA
fluorescein diacetate |
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