Regulation of ammonium and potassium uptake by glutamine and asparagine in Pisum arvense plants |
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Authors: | Genowefa Kubik-Dobosz Józef Buczek |
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Institution: | (1) Department of Plant Physiology, Institute of Botany, Wrocław University, Kanonia 6/8, 50-328 Wrocław, Poland |
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Abstract: | Pisum arvense plants were subjected to 5 days of nitrogen deprivation. Then, in the conditions that increased or decreased the root glutamine
and asparagine pools, the uptake rates of 0.5 mM NH4
+ and 0.5 mM K+ were examined. The plants supplied with 1 mM glutamine or asparagine took up ammonium and potassium at rates lower than those
for the control plants. The uptake rates of NH4
+ and K+ were not affected by 1 mM glutamate. When the plants were pre-treated with 100 μM methionine sulphoximine, an inhibitor of
glutamine synthesis, the efflux of NH4
+ from roots to ambient solution was enhanced. On the other hand, exposure of plants to methionine sulphoximine led to an increase
in potassium uptake rate. The addition of asparagine, glutamine or glutamate into the incubation medium caused a decline in
the rate of NH4
+ uptake by plasma membrane vesicles isolated from roots of Pisum arvense, whereas on addition of methionine sulphoximine increased ammonium uptake. The results indicate that both NH4
+ and K+ uptake appear to be similarly affected by glutamine and asparagine status in root cells.
The research was supported by grant of KBN No. 6PO4C 068 08 |
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Keywords: | ammonium asparagine glutamine methionine sulphoximine plasma membrane vesicles Pisum arvense potassium uptake |
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