Evidence for sterol carrier protein2-like activity in hepatic, adrenal and ovarian cytosol |
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| Authors: | R Chanderbhan T Tanaka J F Strauss D Irwin B J Noland T J Scallen G V Vahouny |
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| Institution: | 1. Department of Biochemistry, George Washington University School of Medicine and Health Sciences, Washington, D.C. 20037 USA;2. Department of Obstetrics and Gynecology. University of Pennsylvania Philadelphia, PA. 19104 USA;3. Department of Biochemistry, University of New Mexico School of Medicine, Albuquerque, N.M. 87131 USA |
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| Abstract: | Purified sterol carrier protein2 (SCP2) from rat liver stimulated utilization of endogenous cholesterol for pregnenolone synthesis by adrenal mitochondria. Cytosolic preparations of rat liver, adrenal and luteinized ovary were also stimulatory in mitochondrial pregnenolone synthesis to different extents. Treatment of all preparations with rabbit anti-rat SCP2 IgG neutralized the stimulatory effects, and immunoprecipitated proteins gave similar patterns on SDS-gradient polyacrylamide gel electrophoresis. Treatment with rabbit pre-immune IgG had no effect on these parameters. Thus, proteins which are immunochemically compatible with hepatic SCP2 appear to be present in steroidogenic tissues and may play a role in control of mitochondrial cholesterol side chain cleavage activity. |
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