Biosynthesis of poly(3-hydroxybutyrate-<Emphasis Type="Italic">co</Emphasis>-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic <Emphasis Type="Italic">Burkholderia</Emphasis> sp. IS-01 |
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Authors: | Do Young Kim Doo-Sang Park Soon Bum Kwon Moon Gyu Chung Kyung Sook Bae Ho-Yong Park Young Ha Rhee |
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Institution: | (1) College of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, 110016, China;(2) Present address: Department of Life Sciences, Liaoning University, Shenyang, 110036, China |
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Abstract: | Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case,
the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated
by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA
synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino
acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity). |
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