首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Membrane‐Based Approach for the Downstream Processing of Influenza Virus‐Like Particles
Authors:Sofia B Carvalho  Ricardo J S Silva  Mafalda G Moleirinho  Brbara Cunha  Ana S Moreira  Alex Xenopoulos  Paula M Alves  Manuel J T Carrondo  Cristina Peixoto
Institution:Sofia B. Carvalho,Ricardo J. S. Silva,Mafalda G. Moleirinho,Bárbara Cunha,Ana S. Moreira,Alex Xenopoulos,Paula M. Alves,Manuel J. T. Carrondo,Cristina Peixoto
Abstract:Currently, marketed influenza vaccines are only efficient against homologous viruses, thus requiring a seasonal update based on circulating subtypes. This constant reformulation adds several challenges to manufacturing, particularly in purification due to the variation of the physicochemical properties of the vaccine product. A universal platform approach capable of handling such variation is therefore of utmost importance. In this work, a filtration‐based approach is explored to purify influenza virus‐like particles. Switching from adsorptive separation to size‐based purification allows overcoming the differences in retention observed for different influenza strains. The proposed process employs a cascade of ultrafiltration and diafiltration steps, followed by a sterile filtration step. Different process parameters are assessed in terms of product recovery and impurities’ removal. Membrane chemistry, pore size, operation modes, critical flux, transmembrane pressure, and permeate control strategies are evaluated. After membrane selection and parameter optimization, concentration factors and diafiltration volumes are also defined. By optimizing the filtration mode of operation, it is possible to achieve product recoveries of approximately 80%. Overall, the process time is decreased by 30%, its scalability is improved, and the costs are reduced due to the removal of chromatography and associated buffer consumptions, cleaning, and its validation steps.
Keywords:filtration  influenza  tangential flow filtration  vaccines  virus‐like particles
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号