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Abstract: | The common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) is one of the most destructive phytophagous pests of crops. Body coloration affects how animals interact with the environment, and pigmentation also influences behavior and immunity. To explore the mechanisms that drive pigmentation, the CRISPR/Cas9 genome editing system was applied to target the ebony gene in the non‐model insect S. litura. The mutants showed the typically deep coloration during the pupal and adult stage. This study clearly demonstrates the function of ebony in the body coloration and also provides a potentially useful marker gene for functional studies in S. litura as well as other lepidopteran pests (see pages 1011–1019). Photo provided by Hong‐Lun Bi. |
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