Expression of endogenous retrovirus ev/J <Emphasis Type="Italic">gp85</Emphasis> gene and analysis of its immunoreactivity in comparison with exogenous viral protein |
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Authors: | Yu-ying Yang Ai-jian Qin Xiong-yan Liang Shu-mei Tong |
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Institution: | (1) Colloge of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China;(2) College of Animal Science, Yangtzeu University, Jingzhou, 434023, China |
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Abstract: | The envelope gene gp85 of ev/J, a new family of endogenous avian retroviral sequences identified recently, has the most extensive nucleotide sequence
identity ever described with ALV-J avian leukosis virus. This report described expression of ev/J envelope gene gp85 derived from commercial meat-type chicken using the Invitrogen Bac-to-Bac baculovirus expression system. The antigenicity
and immunoreactivity of the recombinant endogenous gp85 gene product (SU) were analyzed by indirect immunofluorescence, Western blot, indirect and blocking Enzyme-Linked ImmunoSorbent
Assay (ELISA) using JE9 monoclonal antibody (MAb) against the envelope protein of ALV-J (ADOL-4817), positive mouse antiserum
against the ev/J gp85 SU and sera from chicken naturally infected with ALV-J. The results showed that the ev/J gp85 SU can bind specifically to JE9 MAb and antiserum from chicken naturally infected with ALV-J, and the binding reactivity
between exogenous ALV-J gp85 SU and natural positive chicken serum against exogenous ALV-J can be blocked by positive mouse serum against the ev/J gp85 SU. It is concluded that recombinant endogenous gp85 gene product (SU) has close immunological relatedness to the envelope protein of exogenous ALV-J (ADOL-4817 and IMC10200 strain).
Foundation items: Natural Science Foundation of China (30460098); China Postdoctoral Science Foundation funded project (2005038585). |
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Keywords: | Avian endogenous retrovirus ev/J Envelope gene gp85 Expression Immunoreactivity |
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