Predator-induced defences in <Emphasis Type="Italic">Daphnia pulex</Emphasis>: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR |
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Authors: | Katina I Spanier Florian Leese Christoph Mayer John K Colbourne Don Gilbert Michael E Pfrender Ralph Tollrian |
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Institution: | (1) Department of Animal Ecology, Evolution and Biodiversity, Ruhr-University Bochum, D-44780 Bochum, Germany;(2) Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA;(3) The Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA |
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Abstract: | Background The planktonic microcrustacean Daphnia pulex is among the best-studied animals in ecological, toxicological and evolutionary research. One aspect that has sustained interest
in the study system is the ability of D. pulex to develop inducible defence structures when exposed to predators, such as the phantom midge larvae Chaoborus. The available draft genome sequence for D. pulex is accelerating research to identify genes that confer plastic phenotypes that are regularly cued by environmental stimuli.
Yet for quantifying gene expression levels, no experimentally validated set of internal control genes exists for the accurate
normalization of qRT-PCR data. |
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