| 美洲黑貂5-HT1A受体基因多态性与自咬行为的相关性 |
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| 引用本文： | 林宁,白秀娟.美洲黑貂5-HT1A受体基因多态性与自咬行为的相关性[J].动物学杂志,2008,43(6):51-56. |
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| 作者姓名： | 林宁 白秀娟 |
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| 作者单位： | 1. 东北农业大学生命科学学院,哈尔滨,150030
2. 东北农业大学动物科技学院,哈尔滨,150030 |
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| 摘 要： | 以美洲黑貂(Mustela vison)5-羟色胺1A(5-HT1A)受体基因作为影响其自咬行为的可能候选基因,分析该基因对美洲黑貂自咬行为的影响.本实验采用单链构象多态性(PCR-SSCP)方法进行了多态性检测,并对该基因的不同基因型与自咬行为的关系进行分析.结果表明,在美洲黑貂群体中5-HT1A受体基因136位点发生碱基突变(T-G)的SNPs对美洲黑貂自咬行为性状无影响(χ2=1.393 6,P0.2),在287位点发生(C-G)突变对美洲黑貂的自咬行为有一定的影响(χ2=3.769 4,P<0.2).
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| 关 键 词： | 美洲黑貂 5-HT1A受体基因 自咬行为 |
| 收稿时间： | 2008/3/19 0:00:00 |
| 修稿时间： | 2008/9/21 0:00:00 |
The Polymorphism of 5-HT1A Receptor Gene and its Relationship with Self-biting Behavior in American Mink |
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| LIN Ning and BAI Xiu-Juan.The Polymorphism of 5-HT1A Receptor Gene and its Relationship with Self-biting Behavior in American Mink[J].Chinese Journal of Zoology,2008,43(6):51-56. |
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| Authors: | LIN Ning and BAI Xiu-Juan |
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| Institution: | LIN Ning~ BAI Xiu-Juan~ ( College of Life Science,Northeast Agricultural University,Harbin 150030,College of Animal Science,Northeast Agriculture University,China) |
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| Abstract: | We report the effects of candidate gene 5-HT_ 1A Receptor (5-HT_ 1A R) gene on self-biting of American Mink(Mustela vison)in this paper.Single nucleotide polymorphisms (SNPs) of the 5-HT_ 1A receptor gene were identified and genotyped by polymerase chain reaction followed by single strand conformation polymorphism (PCR-SSCP) analysis.Correlation analysis between 5-HT_ 1A R polymorphisms and self-biting behavior was carried out using Chi-square test.Results show that the genotypes of 5-HT_ 1A R gene at nucle... |
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| Keywords: | PCR-SSCP |
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