An evaluation of sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the identification of microsporidia |
| |
Authors: | Douglas A Streett John D Briggs |
| |
Institution: | Department of Entomology, The Ohio State University, 1735 Neil Avenue, Columbus, Ohio 43210 USA |
| |
Abstract: | Microsporidian spore polypeptides separated with sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) can be used to identify isolates of microsporidia. The spore polypeptides separated with SDS/PAGE provided unique, reproducible electrophoretic profiles which were not influenced by host species or the temperature at which the host larvae were maintained for development. Furthermore, host proteins were not detected in electrophoretic profiles of the spore polypeptides. Spore mixtures of two microsporidian species can be detected when the spore polypeptides of either or both species have been previously separated with SDS/PAGE. |
| |
Keywords: | Sodium dodecyl sulfate polyacrylamide gel electrophoresis microsporidia identification spore polypeptides |
本文献已被 ScienceDirect 等数据库收录! |