The estimation of the oxidized and reduced forms of the nicotinamide nucleotides |
| |
| Authors: | A. L. Greenbaum J. B. Clark Patricia McLean |
| |
| Affiliation: | Department of Biochemistry, University College London, Gower Street, London, W.C. 1;Courtauld Institute of Biochemistry, The Middlesex Hospital Medical School, London, W. 1 |
| |
| Abstract: | 1. A method is described for the determination of the oxidized and reduced forms of the nicotinamide nucleotides by measuring the rate of the oxygen uptake with an oxygen electrode in a system in which the nucleotide acts as the rate-limiting carrier in a cyclic system. 2. The method permits the measurement of quantities as low as 0·02μg. of NAD+ or NADH or 0·01μg. of NADP+ or NADPH. 3. The method permits the measurement of the nucleotides in extracts that contain non-specific reducing substances, coloured compounds or fluorescent materials, e.g. green leaves. 4. The results obtained by the present method are compared with those reported in the literature. |
| |
| Keywords: | |
|
|
