Juvenile hormone III biosynthesis by the larval corpora allata of Manduca sexta |
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| Authors: | Noelle A Granger Sheryl M Niemiec Lawrence I Gilbert Walter E Bollenbacher |
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| Institution: | 1. Department of Anatomy, School of Medicine, University of North Carolina, Chapel Hill, NC 27514, U.S.A.;2. Department of Zoology, University of North Carolina, Chapel Hill, NC 27514, U.S.A.;3. Department of Biological Sciences, Northwestern University, Evanston, IL 60201, U.S.A. |
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| Abstract: | A radioimmunoassay (RIA) for juvenile hormone III has been established which quantifies the biosynthesis of this hormone in vitro by the corpora allata of larvae and pupae of the tobacco hornworm, Manduca sexta. The specificity of the RIA for homologues and metabolites of juvenile hormone III was determined and it was found that the antibody was specific for juvenile hormone III and its acid. The juvenile hormone III RIA activity synthesized in vitro by corpora allata from day-5 last-instar larvae was identified as juvenile hormone III by high pressure liquid chromatography. The kinetics of hormone synthesis by corpora allata from selected stages during larval-pupal development revealed differential rates of synthesis, suggesting that juvenile hormone III may have a hormonal function in the larva and that regulation of its synthesis may occur. The significance of these developmental fluctuations in rates of juvenile hormone III synthesis by the corpora allata is discussed in relation to the haemolymph titres of the hormone. |
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| Keywords: | Corpora allata juvenile hormone III larval-pupal development |
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